Monoclonal antibody enzyme-linked immunosorbent assay for identification of K99-positive Escherichia coli isolates from calves
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منابع مشابه
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متن کاملPreparation of monoclonal antibody and development of enzyme-linked immunosorbent assay specific for Escherichia coli O157 in foods.
OBJECTIVE To prepare monoclonal antibodies (MAb) and antisera specific for Escherichia coli (E. coli) O157, and to develop a sandwich enzyme-linked immunosorbent assay (ELISA) to detect E. coli O157 in foods. METHODS Spleen cells from BALB/c mice immunized with the somatic antigen of E. coli O157:H7 were fused with murine Sp2/0 myeloma cells. The hybridoma cell line specific for E. coli O157 ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1984
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.19.4.498-501.1984